Preparation and characterization of caffeic acid grafted chitosan/CPTMS hybrid scaffolds

In this study, preparation and characterization of caffeic acid grafted chitosan (CTS)/(3-chloropropyl)trimethoxysilane (CPTMS) hybrid scaffolds were investigated. The grafting of caffeic acid onto CSC1.0 (CTS/CPTMS hybrid scaffold prepared in weight ratio of 1:1) not only increased its compressive strength but also enhanced its antibacterial, antioxidant and anti-cancer property. The caffeic acid grafted scaffolds exhibited higher anti Staphylococcus aureus and scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) property than CSC1.0 did. The compressive strength of the caffeic acid grafted scaffolds is 0.54∼0.65 MPa, which was higher than that of CTS scaffold (0.14 MPa) and CSC1.0 (0.33 MPa). In culturing human osteosarcoma UMR-106 cells, the caffeic acid grafted scaffolds showed inhibitions on cell growth, alkaline phosphatase activity and cell attachment of UMR-106 cells. Caffeic acid grafting approach could be very promising for chitosan scaffolds to be used in hard-tissue engineering application.