Antioxidant activity of levan coated cerium oxide nanoparticles

• Levan coated cerium oxide nanoparticles (LCNPs) were synthesized by one pot and green synthesis system.
• LCNPs with the enhanced antioxidant activity were successfully synthesized and characterized.
• The morphology of nanoparticles was uniformly spherical and hydrodiameter of the nanoparticles was approximately 40 nm.
• LCNPs have water solubility and biocompatibility.
• LCNPs show high antioxidant activity in neutral environment.

Levan coated cerium oxide nanoparticles (LCNPs) with the enhanced antioxidant activity were successfully synthesized and characterized. Levan and their derivatives are attractive for biomedical applications attributable to their antioxidant, anti-inflammation and anti-tumor properties. LCNPs were synthesized using the one-pot and green synthesis system with levan. For production of nanoparticles, levan plays a role as a stabilizing and reducing agent. Fourier transform infrared spectroscopy (FT-IR) analysis showed that LCNPs successfully synthesized. The morphology and size of nanoparticles were confirmed by transmission electron microscopy (TEM) and dynamic light scattering (DLS). LCNPs have good water solubility and stability. The conjugation of levan with cerium oxide nanoparticles improved antioxidant activity. Moreover the level of ROS was reduced after treatment of LCNPs to H2O2 stimulated NIH3T3 cells. These results demonstrate that the LCNPs are useful for applying of treatment of ROS induced diseases.