Effects of chitooligosaccharides on rabbit neutrophils in vitro

The effects of chitooligosaccharides(COS) on resting and PMA-activated neutrophils were estimated. MTT assays, NO estimation and superoxide detection revealed that chitooligosaccharides at concentrations of 25, 50, 75, and 100 μg/ml increased the viability, ability to produce reactive oxygen intermediates and nitrogen intermediates of resting neutrophils. Superoxide detection, degranulation assay and adhesion assay suggested that chitooligosaccharides at concentrations from 50 to 150 μg/ml reduced PMA-induced activation of neutrophils.