Extraction optimization and antinociceptive activity of (1⟶3)-β-d-glucan from Rhodotorulamucilaginosa

• Rhodotorulamucilaginosa was used to obtain glucan.
• Biopolymers were purified by molecular exclusion chromatography using a column of Sephacryl S-200.
• Glucan was characterized using infrared and nuclear magnetic resonance spectroscopy.
• Infrared and nuclear magnetic resonance spectroscopy shows that is a (1⟶3)-β-d-glucan.
• Biological activity of (1⟶3)-β-d-glucan was evaluated using different tests of nociception.

β-d-glucans are polymers of d-glucose monomers found in the cell walls of many bacteria, plants, fungi and yeasts. A variety of β-d-glucans differing in structures have been isolated from various sources and their biological activity to be regulated by various structural factors, such as the primary structure, molecular weight, solubility, and conformation. This study investigated the effect of extraction time and temperature on the yield of β-d-glucan produced by Rhodotorulamucilaginosa. A statistical Doehlert design was applied to determine the important effects and interactions of these independent variables on the yield of β-d-glucan, the dependent variable. Significant models were obtained. The best yield was of 25% obtained after 128 min of extraction in a temperature of 72 °C. The polysaccharides were characterized as (1⟶3)-β-d-glucan by methods spectroscopic (FT-IR, 1HNMR and 13CNMR). In addition, the antinociceptive effect was evaluated using different experimental tests (acetic acid-induced writhing test, formalin test and tail immersion test). The (1⟶3)-β-d-glucan showed a potent peripheral antinociceptive effect, possibly by the inhibition of inflammatory mediators.

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