کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1386663 982524 2011 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MSn of the six isomers of (GlcN)2(GlcNAc)2 aminoglucan tetrasaccharides (diacetylchitotetraoses): Rules of fragmentation for the sodiated molecules and application to sequence analysis of hetero-chitooligosaccharides
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
MSn of the six isomers of (GlcN)2(GlcNAc)2 aminoglucan tetrasaccharides (diacetylchitotetraoses): Rules of fragmentation for the sodiated molecules and application to sequence analysis of hetero-chitooligosaccharides
چکیده انگلیسی

The six possible isomers of di-N-acetylchitotetraoses [AADD, ADDA, ADAD, DADA, DAAD, and DDAA, where D stands for 2-amino-2-deoxy-β-d-glucose (GlcN) and A for 2-acetamido-2-deoxy-β-d-glucose (GlcNAc)] were analyzed by ESI(+)-MSn. Collision induced dissociation via MSn experiments were performed for the sodiated molecules of m/z 769 [M+Na]+ for each isomer, and fragments were generated mainly by glycosidic bond and cross-ring cleavages. Rules of fragmentation were then established. A reducing end D residue yields the O,2A4 cross-ring [M−59+Na]+ fragment of m/z 710 as the most abundant, whereas isomers containing a reducing end A prefer to lose water to form the [M−18+Na]+ ion of m/z 751, as well as abundant O,2A4 cross-ring [M−101+Na]+ fragments of m/z 668 and B3 [M−221+Na]+ ions of m/z 548. MS3 of C- and Y-type ions shows analogous fragmentation behaviour that allows identification of the reducing end next-neighbour residue. Due to gas-phase anchimeric assistance, B-type cleavage between the glycosidic oxygen and the anomeric carbon atom is favoured when the glycon is an A residue. Relative ion abundances are generally in the order B ≫ C > Y, but may vary depending on the next neighbour towards the non-reducing end. These fragmentation rules were used for partial sequence analysis of hetero-chitooligosaccharides of the composition D2A3, D3A3, D2A4, D4A3, and D3A4.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 84, Issue 2, 1 March 2011, Pages 713–726
نویسندگان
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