کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1387088 | 982545 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Enhanced biomass production of Pycnoporus sanguineus and alterations in the physiochemical properties of its polysaccharides Enhanced biomass production of Pycnoporus sanguineus and alterations in the physiochemical properties of its polysaccharides](/preview/png/1387088.png)
The purpose of this research was to physiochemically characterize the expression profiles of polysaccharides produced by Pycnoporus sanguineus using different cultural conditions including media utilizing different carbohydrate sources and pH values. Polysaccharides were characterized by size-exclusion chromatography (SEC) and high-performance anion-exchange chromatography (HPAEC). The maximum mycelial growth reached a value of 16.52 ± 1.03 g/l when P. sanguineus was fed 20 g/l sucrose with 20 g/l potato dextrose broth (PDB). Medium-high-molecular-weight polysaccharides (50–100 kDa) were largely synthesized by glucose feeding. The synthesis of low-molecular-weight polysaccharides (<30 kDa) decreased when the pH of the medium increased. Fucose, galactose, glucose, and mannose were the dominant sugars in the P. sanguineus polysaccharide mixture. We determined correlations between sugar components in the polysaccharides and the type of carbon source in the medium. Feeding with sucrose or glucose resulted in a direct dosage effects on the fucose and mannose components of the polysaccharides.
Journal: Carbohydrate Polymers - Volume 83, Issue 2, 10 January 2011, Pages 796–801