Extraction, characterization of the polysaccharide extracts from Se-enriched G. lucidum (Se-GLP) and its inhibition against oxidative damage in ischemic reperfusion mice

By analyses using high-performance liquid chromatography (HPLC), the polysaccharide extracts from Se-enriched Ganoderma lucidum (Se-GLP) was comprised mainly of glucose, xylose, arabose and mannose in the molar ratio of 1:4.3:0.7:0.18. the infrared (IR) spectra of the Se-GLP displayed a broad stretching intense characteristic peak at around 3402 and 3105 cm−1 for the hydroxyl group, and one weak C–H stretching bands at 2927 and 2702 cm−1. The peaks at 1163, 947 and 890 cm−1 were characteristic of xylose, arabose and β-d-glucose, respectively. The results indicated that glucose and xylose were the major monoses constructing the backbones of Se-GLP. Then, the protective effects of the polysaccharide extracts from Se-enriched G. lucidum (Se-GLP) against oxidative damage were evaluated in heart and liver of ischemic reperfusion mice. The results showed that the treatment of Se-GLP significantly lowered the ischemic reperfusion-induced serum levels of malondialdehyde (MDA) and intercellular adhesion molecule-1 (ICAM-1). The heart and serum content of GSH, and activities of Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (TAOC) that were reduced by ischemic reperfusion were brought back to control levels by the supplement of Se-GLP. Therefore, the results of this study suggest that Se-GLP could protect heart against the ischemic reperfusion-induced oxidative damage in mice.