کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1387287 982565 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Carbohydrates present in the glycoprotein from conidia of the opportunistic pathogen Scedosporium prolificans
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Carbohydrates present in the glycoprotein from conidia of the opportunistic pathogen Scedosporium prolificans
چکیده انگلیسی

Hot aqueous extraction of conidia of Scedosporium prolificans gave a heterogeneous glycoprotein (RMP-Sp-Coni) with 41% protein and 2MeRha, Rha, Ara, Man, Gal, Glc, and GlcNH2 in a 2:18:3:47:9:15:6 M ratio, the first report of 2-O-methylrhamnose in fungi. Methylation analysis showed nonreducing end- (10%), 2-O- (11%), and 3-O-substituted Rhap (7%), nonreducing end- (8%), 2-O- (12%), 3-O- (16%), and 2,6-di-O-substituted Manp (9%), nonreducing end- (4%), 3-O- (7%), and 4-O-substituted Glcp (7%), and nonreducing end-units of Galp (9%). Mild reductive β-elimination of RMP-Sp-Coni cleaved O-linked structures to give a mixture of oligosaccharides, of which 2MeRha capping groups were present in 2MeRhaRha2Hex2Hex-ol, 2MeRhaRha2Hex-Hex-ol, 2MeRhaRha2HexHex-ol, and 2MeRhaRha2Hex-ol (ESI-MS–MS). The mixture was fractionated by Biogel P-2 column chromatography and the two predominant isolates were β-d-Galp-(1 → 6)-[2Me-α-l-Rhap-(1 → 3)-α-l-Rhap-(1 → 3)-α-d-Manp-(1 → 2)]-d-Man-ol, and another lacking the β-Galp unit. Neither was formed from mycelial glycoprotein, although β-d-Galp-(1 → 6)-[α-l-Rhap-(1 → 3)-α-l-Rhap-(1 → 3)-α-d-Manp-(1 → 2)]-d-Man-ol was a common component.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 79, Issue 4, 17 March 2010, Pages 927–932
نویسندگان
, , , , , , , , ,