کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1387590 1500838 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzymatic synthesis of hyaluronan hybrid urinary trypsin inhibitor
ترجمه فارسی عنوان
سنتز آنزیمی مهارکننده تریپسین ادرار ترکیبی هیالورونان
کلمات کلیدی
مهار کننده تریپسین ادرار، گلیکواسامینو گلیکان، کندرویتین سولفات، هیالورونان، هیالورونیداز، ترگلسی کلاسیک
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی


• Hyaluronan hybrid urinary trypsin inhibitor (HA-UTI) was enzymatically synthesized.
• HA-UTI bound to hyaluronan binding protein.
• HA-UTI retained high trypsin inhibitor activity similar to native UTI.
• HA-UTI had lower hyaluronidase inhibitor activity than native UTI.
• HA-UTI interaction with phosphatidylcholine was stronger than that of native UTI.

Human urinary trypsin inhibitor is a proteoglycan that has a single low-sulfated chondroitin 4-sulfate chain at the seryl residue in position 10 of the core protein as a glycosaminoglycan moiety, and is used as an anti-inflammatory medicine based on the protease inhibitory activity of the core protein. However, the functions of the glycosaminoglycan moiety have not yet been elucidated in detail. In the present study, the glycosaminoglycan chains of a native urinary trypsin inhibitor were remodeled to hyaluronan chains, with no changes to the core protein, using transglycosylation as a reverse reaction of the hydrolysis of bovine testicular hyaluronidase, and the properties of the hybrid urinary trypsin inhibitor were then analyzed. The trypsin inhibitory activitiy of the hyaluronan hybrid urinary trypsin inhibitor was similar to that of the native type; however, its inhibitory effect on the hydrolysis of hyaluronidase were not as strong as that of the native type. This result demonstrated that the native urinary trypsin inhibitor possessed hyaluronidase inhibitory activity on its chondroitin sulfate chain. The hyaluronan hybrid urinary trypsin inhibitors obtained affinity to a hyaluronan-binding protein not exhibited by the native type. The interactions between the hyaluronan hybrid urinary trypsin inhibitors and phosphatidylcholine (abundant in the outer layer of plasma membrane) were stronger than that of the native type. Hyaluronan hybrid urinary trypsin inhibitors may be useful for investigating the functions of the glycosaminoglycan chains of urinary trypsin inhibitors and hyaluronan, and our hybrid synthesizing method may be used widely in research for future medical applications.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 413, 2 September 2015, Pages 129–134
نویسندگان
, , , , ,