A new, quick, highly sensitive ultramicro-analysis method for the identification of fructose removed from fructofuranosyl-containing gluco-oligosaccharides by ESI-CID-MS/MS

• Determination of characteristic fragmentations.
• Diagnostic tool to identify if the fructose terminal is removed from oligosaccharides.
• Created a calibration curve for quantifying the degree of fructose removal from oligosaccharides.

An efficient, highly sensitive, and ultramicroscale analytical method for the identification of fructose removed from fructofuranosyl-containing gluco-oligosaccharides, including malto-oligosyl fructofuranosides and oligomeric (1→2)-α-d-glucopyranosyl-(1→2)-β-d-fructofuranosides by ESI-CID-MS/MS has been developed with proven applications far superior to the existing method using NMR. With the established principle of diagnostic fragmentation by ESI-CID-MS/MS, the terminal saccharide (either glucose or fructose) can be readily and unambiguously determined at high sensitivity without a tedious derivatization process. Detection of the A-type fragmentation 0,4A-h type ion, and 0,2A type ion are useful as a diagnostic fragmentation tool to identify whether fructose terminal is removed from oligosaccharides. It will facilitate the efficient production of suitable oligosaccharide microarrays crucial for studies on carbohydrate–protein interaction in seeking functional carbohydrates.

Identification of the removal of fructose from fructofuranosyl-containing glucooligosaccharides based on the regular pattern of negative ESI-CID-MS/MS product-ion.Figure optionsDownload as PowerPoint slide