Structure and gene cluster organization of the O-antigen of Providencia alcalifaciens O45:H25

• Lipopolysaccharide of Providencia alcalifaciens O45:H25 was degraded with mild acid.
• Structure of the O-polysaccharide obtained was established by chemical and NMR methods.
• The O-polysaccharide was found to be in agreement with the O-antigen gene cluster.

O-Polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O45:H25 and studied by sugar analysis, Smith degradation, and 1H and 13C NMR spectroscopy. The following structure of the pentasaccharide repeat of the O-polysaccharide was established:Figure optionsDownload as PowerPoint slideThe O-antigen gene cluster of P. alcalifaciens O45 was sequenced and found to be in full agreement with the O-polysaccharide structure established.

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