Carbohydrate-functionalized collagen matrices: design and characterization of a novel neoglycosylated biomaterial

• Molecular dynamics simulations on neoglycosylated collagen were performed.
• We performed reductive amination on collagen matrices.
• Lactose was covalently linked at lysine residues.
• The morphology of collagen matrices was assessed by AFM.
• ELLA showed effective biodecoration of collagen matrices.

Collagen matrices have been neoglycosylated with lactose by reductive amination at lysine side chains. AFM analysis highlights that the chemical does not affect molecular assembly into fibrils. Moreover, ELLA biochemical assays show that the glycan moiety is efficiently exposed on the matrix surface for receptor recognition.

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