کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1387902 1500863 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structure elucidation and gene cluster annotation of the O-antigen of Escherichia coli O39; application of anhydrous trifluoroacetic acid for selective cleavage of glycosidic linkages
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Structure elucidation and gene cluster annotation of the O-antigen of Escherichia coli O39; application of anhydrous trifluoroacetic acid for selective cleavage of glycosidic linkages
چکیده انگلیسی


• Structure of the O-polysaccharide of Shiga toxin-producing E. coli O39 was established.
• Solvolysis with trifluoroacetic acid was applied to cleave selectively the rhamnosidic linkage.
• The O-antigen gene cluster of E. coli O39 has been sequenced.

O-Polysaccharide (O-antigen) accompanied by a minor mannan was isolated from the lipopolysaccharide of Escherichia coli O39 and studied by component analyses, methylation, Smith degradation, mass spectrometry, and 1D and 2D NMR spectroscopy. In addition, a new approach, solvolysis with anhydrous trifluoroacetic acid, was applied to cleave selectively the rhamnosidic linkage. The following structure of the O-polysaccharide was established:Figure optionsDownload as PowerPoint slide where d-Qui4N(R3Hb) indicates 4,6-dideoxy-4-[(R)-3-hydroxybutanoylamino]-d-glucose. The O-antigen gene cluster of E. coli O39 has been sequenced. The gene functions were tentatively assigned by a comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 388, 31 March 2014, Pages 30–36
نویسندگان
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