کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1388261 | 982778 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Improved isolation of glucuronan from algae and the production of glucuronic acid oligosaccharides using a glucuronan lyase Improved isolation of glucuronan from algae and the production of glucuronic acid oligosaccharides using a glucuronan lyase](/preview/png/1388261.png)
A new source for the production of bioactive glucuronic acid oligosaccharides (GlcUAOs) from the depolymerization of green seaweed Ulva lactuca glucuronan (Algal glucuronan) has been investigated. Algal glucuronan purification was optimized by the acidic precipitation method which allowed us to separate the polysaccharide mixture extracted from the cell wall of Ulvalactuca using hot water containing sodium oxalate. A series of the GlcUAOs were obtained by enzyme degradation of algal glucuronan with a glucuronan lyase (GL) isolated from Trichoderma strain. The putative bioactive GlcUAOs generated were then purified by size-exclusion chromatography in gram quantity and characterized by 1H/13C NMR spectroscopy and ESI-Q/TOF-mass spectrometry.
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Journal: Carbohydrate Research - Volume 344, Issue 13, 8 September 2009, Pages 1670–1675