Structure of the O-polysaccharide of Escherichia coli O87

• The O-specific polysaccharide was isolated from the lipopolysaccharide of Escherichia coli O87.
• The structure of the tetrasaccharide repeat of the O-polysaccharide was established.
• The structure is consistent with putatively assigned functions of the O-antigen biosynthesis genes.

The following structure of the O-polysaccharide of Escherichia coli HS1/2 serving as a primary receptor for bacteriophage DT57-12 was elucidated by sugar analysis along with 1D and 2D 1H and 13C NMR spectroscopy:Figure optionsDownload as PowerPoint slideThis structure is shared by E. coli O87 type strain. Putatively assigned functions of genes in the O-antigen gene cluster of E. coli O87 are consistent with the O-polysaccharide structure established.

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