کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1388325 982783 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
چکیده انگلیسی

The novel low-molecular-weight chitosan polysulfate (MW 5120–26,200 Da) was prepared using the depolymerization of chitosan with papain (EC. 3.4.22.2). The sulfonation of depolymerized products was performed using chlorosulfonic acid in N,N-dimethylformamide under semi-heterogeneous conditions. The structures of the products were characterized by FTIR, 13C NMR, and 1H NMR (1D, 2D NMR) spectroscopy. The present study sheds light on the mechanism of anticoagulant activity of chitosan polysulfate. Anticoagulant activity was investigated by an activated partial thromboplastin assay, a thrombin time assay, a prothrombin time assay, and thrombelastography. Surface plasmon resonance also provided valuable data for understanding the relationship between the molecular binding of sulfated chitosan to two important blood clotting regulators, antithrombin III and heparin cofactor II. These results show that the principal mechanism by which this chitosan polysulfate exhibits anticoagulant activity is mediated through heparin cofactor II and is dependent on polysaccharide molecular weight.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 344, Issue 10, 6 July 2009, Pages 1190–1196
نویسندگان
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