کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1388441 | 982793 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression, purification and characterization of endo-type chitosanase of Aspergillus sp. CJ22-326 from Escherichia coli
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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چکیده انگلیسی
An endo-chitosanase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an endo-chitosanase activity during viscosimetric assay and TLC analysis. The enzyme had higher chitosanolytic activity than previously reported fungal chitosanases.
The cDNA encoding endo-chitosanase from Aspergillus sp. CJ22-326 was cloned, expressed and its product was purified from E.coli. Chitosanase activity of the recombinant protein was much higher than other heterologous expression fungal proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 343, Issue 17, 24 November 2008, Pages 3001–3004
Journal: Carbohydrate Research - Volume 343, Issue 17, 24 November 2008, Pages 3001–3004
نویسندگان
Songlin Li, Liang Chen, Chen Wang, Wenshui Xia,