کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1388441 982793 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression, purification and characterization of endo-type chitosanase of Aspergillus sp. CJ22-326 from Escherichia coli
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Expression, purification and characterization of endo-type chitosanase of Aspergillus sp. CJ22-326 from Escherichia coli
چکیده انگلیسی

An endo-chitosanase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an endo-chitosanase activity during viscosimetric assay and TLC analysis. The enzyme had higher chitosanolytic activity than previously reported fungal chitosanases.

The cDNA encoding endo-chitosanase from Aspergillus sp. CJ22-326 was cloned, expressed and its product was purified from E.coli. Chitosanase activity of the recombinant protein was much higher than other heterologous expression fungal proteins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 343, Issue 17, 24 November 2008, Pages 3001–3004
نویسندگان
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