The N-glycosylation pattern of Caenorhabditis elegans

Determining the exact nature of N-glycosylation in Caenorhabditis elegans, a nematode worm and genetic model organism, has proved to have been an unexpected challenge in recent years; a wide range of modifications of its N-linked oligosaccharides have been proposed on the basis of structural and genomic analysis. Particularly mass spectrometric studies by a number of groups, as well as the characterisation of recombinant enzymes, have highlighted those aspects of N-glycosylation that are conserved in animals, those which are seemingly unique to this species and those which are shared with parasitic nematodes. These data, of importance for therapeutic developments, are reviewed.

Mass spectrometry of the glycans of the nematode worm Caenorhabditis elegans shows the presence of unusual structures. Shown are pictures of a worm expressing a green fluorescent protein under control of a hexosaminidase promoter, an MS–MS spectrum and a model of a novel glycan carrying galactosylated core fucose.Figure optionsDownload as PowerPoint slide