Structural elucidation of a novel water-soluble fructan isolated from Wedelia prostrata

• We purified a novel water-soluble polysaccharide from Wedelia prostrata called WPP60A.
• Homogeneity and molecular weight (2.40 × 103 Da) of WPP60A were determined by HPLC.
• Its structure was established by GC–MS, 1H, 13C, and 2D NMR analysis.
• The repeating unit consists of [β-d-Fruf-(6→2)-β-d-Fruf-].

A new heteropolysaccharide, WPP60A, with a molecular weight of 2.4 kDa, was isolated from Wedelia prostrata by hot water extraction, and purified by DEAE Sepharose fast flow and Sephacryl S-200 high-resolution chromatography. Compositional analysis, and methylation analysis, combined with 1H, 13C NMR spectroscopy including 2D NMR (COSY, TOCSY, HMQC, NOESY, and HMBC) experiments demonstrated that WPP60A was composed of primarily fructose and low glucose. Results indicated that this new heteropolysaccharide consists of a repeating unit with the following structure: Figure optionsDownload as PowerPoint slide

Repeating unit of polysaccharide from Wedelia prostrata.Figure optionsDownload as PowerPoint slide