A new approach for the determination of oligosaccharide structures

A new method for the determination of oligosaccharide chains, known as the D60 one-dimensional TOCSY method is introduced in this paper. The results show that the use of this method enables a more effective coherent long-range magnetic relay transfer compared with that of existing DIPSI-2 and MMDY methods. Further, the method is easy to use and is not sensitive to the error of the pulse width. Without complex z-filtering steps, the high-quality sub-spectrum of pure absorption can be quickly obtained, which facilitates sub-spectroscopy detection for the existence of weak spin-coupling sugar components in the saccharide ring. Glycosides are taken as examples to discuss the characteristics of this method and its application in the determination of oligosaccharides in spectrum peak height.

Pulse sequences for the subspectra of the sugar components of the oligosaccharide chain obtained by applying D60 one-dimensional TOCSY method.Figure optionsDownload as PowerPoint slideHighlights
► The D60 one-dimensional TOCSY for the determination of oligosaccharide chains is introduced.
► This method enables a more effective coherent long-range magnetic relay transfer.
► It is easy to use and is not sensitive to the error of the pulse width.
► The high-quality sub-spectrum of pure absorption can be quickly obtained.
► It facilitates sub-spectroscopy detection for the existence of weak spin coupling saccharide ring.