کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1389230 | 982854 | 2007 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Preparation and characterization of 6I,6n-di-O-(l-fucopyranosyl)-β-cyclodextrin (n = II–IV) and investigation of their functions Preparation and characterization of 6I,6n-di-O-(l-fucopyranosyl)-β-cyclodextrin (n = II–IV) and investigation of their functions](/preview/png/1389230.png)
Three positional isomers of 6I,6n-di-O-(β-l-fucopyranosyl)-cyclomaltoheptaose [6I,6n-di-O-(β-l-Fuc)-β-cyclodextrin, -βCD, n = II–IV] were chemically synthesized using the corresponding authentic compounds, 6I,6n-di-O-(tert-butyldimethylsilyl)-βCD (n = II–IV), as the fucosyl acceptors, and 2,3,4-tri-O-acetyl-l-fucopyranosyl trichloroacetimidate as the fucosyl donor. Their structures were analyzed by HPLC, MS, and NMR spectroscopy. The hemolytic activities of l-Fuc-βCDs were lower than that of βCD, while the solubilities of these branched CDs in water were much higher than that of βCD. The molecular interaction between these compounds and the fucose-binding lectin Aleuria aurantia lectin (AAL) was investigated using an optical biosensor based on a surface plasmon resonance (SPR) technique. The order of binding affinity, as a function of the fucose-binding position, was 6I,6IV- > 6I,6III- > 6I,6II-di-O-(β-l-Fuc)-βCD > 6-O-(β-l-Fuc)-βCD.
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Journal: Carbohydrate Research - Volume 342, Issue 15, 5 November 2007, Pages 2173–2181