کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1389310 982863 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Selective ketopentose analysis in concentrate carbohydrate syrups by HPLC
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Selective ketopentose analysis in concentrate carbohydrate syrups by HPLC
چکیده انگلیسی

A new high performance liquid chromatographic (HPLC) method is described for the selective determination of small quantities of ketoses obtained by the action of immobilized isomerases on wheat bran hydrolysates, in the concentrated syrups of the corresponding glucose, arabinose, and xylose. This method uses MilliQ water instead of dilute sulfuric acid as a mobile phase on an Aminex HPX-87H column. Excellent discrimination between xylulose and ribulose was achieved. Selective detection of ketoses was made possible by the much higher UV absorbance at 210 nm. The sensitivity limit is 0.5 g/L for d-xylulose and l-ribulose. The response is linear up to a 20 g/L ketose concentration regardless of the presence of less than 50 g/L of d-xylose or l-arabinose.

This paper describes a chromatographic method for the detection of low ketose concentrations in a complex syrup containing high concentration of carbohydrates such as glucose and pentoses by UV detection at 210 nm.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 342, Issue 6, 7 May 2007, Pages 859–864
نویسندگان
, , ,