Expression, purification, and characterization of exo-β-d-glucosaminidase of Aspergillus sp. CJ22-326 from Escherichia coli

An exo-β-d-glucosaminidase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an exo-chitosanase activity in a viscosimetric assay and TLC analysis. This is the first report on cloning of a gene encoding an Aspergillus sp. exo-β-d-glucosaminidase.

The cDNA encoding exo-β-d-glucosaminidase from Aspergillus sp. CJ22-326 was cloned, expressed and its product was purified from Escherichia coli. This is the first report on cloning of the gene encoding Aspergillus sp. exo-β-d-glucosaminidase.Figure optionsDownload as PowerPoint slide