کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1389637 | 982904 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Production of oligoglucuronans using a monolithic enzymatic microreactor Production of oligoglucuronans using a monolithic enzymatic microreactor](/preview/png/1389637.png)
A glucuronan lyase (EC 4.2.2.14) was immobilized on a monolithic Convective Interaction Media (CIM®) disk. The immobilization yield was equal to 29% of the initial activity and 35% of the initial protein amount. Degradations of three glucuronans with various O-acetylation degrees were investigated and compared with degradations using free enzyme. The immobilized glucuronan lyase was inhibited by the O-acetylation degree like the free enzyme. 1H NMR analyses were used to study the O-acetylation degree of oligoglucuronans and demonstrated that the average degrees of polymerization were inclusive between 4 and 13 after 24 h of degradation. This first immobilization of a glucuronan lyase constitutes a new tool to produce oligoglucuronans.
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Journal: Carbohydrate Research - Volume 343, Issue 15, 13 October 2008, Pages 2687–2691