کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1390019 982956 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation of the methyl ester of hyaluronan and its enzymatic degradation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Preparation of the methyl ester of hyaluronan and its enzymatic degradation
چکیده انگلیسی

A methyl ester of hyaluronan in which the carboxyl groups were fully esterified was prepared using trimethylsilyl diazomethane. This derivative, while not depolymerized by hyaluronan lyases or hyaluronan hydrolases, was a substrate for both chondroitin ACI lyase (EC 4.2.2.5) from Flavobacterium heparinum and chondroitin ACII lyase (EC 4.2.2.5) from Arthrobacter aurescens. The major product isolated in these depolymerization reactions was methyl α-l-threo-hex-4-enepyranosyluronate-(1→3)-2-acetamido-2-deoxy-α,β-d-glucopyranoside as determined by 1H NMR spectroscopy and MALDITOF mass spectrometry.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 340, Issue 14, 17 October 2005, Pages 2297–2304
نویسندگان
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