Structure of the O-specific polysaccharide from the marine bacterium Rheinheimera japonica KMM 9513T, containing N-glycosidic bond between monosaccharides

• Structure of the O-polysaccharide of R. japonica КММ 9513T was established.
• The repeating unit of O-polysaccharide is a branched hexasaccharide.
• The N-glycosidic bond was found in O-polysaccharide for the first time.

The O-specific polysaccharide was isolated from the lipopolysaccharide of type strain Rheinheimera japonica KMM 9513T and studied by sugar analysis, Smith degradation, and two-dimensional 1H and 13C NMR spectroscopy including 1H,1H-TOCSY, 1H,1H-COSY, 1H,1H-ROESY, 1H,13C-HSQC, 1H,13C-HMBC, 1H,13C-H2BC and 1H,13C-HSQC-TOCSY experiments. The new structure of the O-specific polysaccharide of R. japonica KMM 9513T containing N-glycosidic bond was established:Figure optionsDownload as PowerPoint slide

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