Complete NMR assignment of a bisecting hybrid-type oligosaccharide transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase

• The nuclear magnetic resonance spectral assignment of a bisecting hybrid-type oligosaccharide, transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase (Endo-M).
• The complete NMR assignment of a bisecting hybrid-type oligosaccharide through 1H- and 13C-NMR, DQF-COSY, HSQC, HMBC, TOCSY, and NOESY experiments.
• The determination of the structure of the glycoside linkage formed by the Endo-M transglycosylation.

This study describes the complete nuclear magnetic resonance (NMR) spectral assignment of a bisecting hybrid-type oligosaccharide 1, transferred by Mucor hiemalis endo-β-N-acetylglucosaminidase (Endo-M). Through 1H- and 13C-NMR, DQF-COSY, HSQC, HMBC, TOCSY, and NOESY experiments, we determine the structure of the glycoside linkage formed by the Endo-M transglycosylation, i.e., the connection between GlcNAc and GlcNAc in oligosaccharide 1.

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