کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1390144 | 1500843 | 2015 | 7 صفحه PDF | دانلود رایگان |

• Gut microbes have evolved selected enzymes to degrade host-derived glycans.
• We reveal novel substrate specificities of two non-homologous lacto-N-biosidases.
• LnbX, a non-classified GH member, acts on Gb5 and globo H oligosaccharides.
• LnbB, a member of GH20, acts on GA1 and Gb5 oligosaccharides.
• These enzymes will provide new tools for glycobiology.
We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids. LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp. longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2′-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively. LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides. This is the first report describing enzymatic release of β-linked GNB from natural substrates. These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids.
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Journal: Carbohydrate Research - Volume 408, 18 May 2015, Pages 18–24