New complete structure of Hafnia alvei clinical isolate strain PCM 2670 semi-rough lipopolysaccharide

• The LPS of Hafnia alvei PCM strain 2670 was isolated and analyzed.
• New core OS structure was established by means of 2D NMR spectroscopy and ESIMS.
• O-Specific PS structure was established by means of 2D NMR spectroscopy.
• Linkage between core OS and O-specific PS was established.

Hafnia alvei strain PCM 2670 is a clinical isolate from a patient with chronic reproductive tract infection. The novel structure of the semi-rough lipopolysaccharide was established with the use of NMR spectroscopy and mass spectrometry as well as immunochemical techniques. According to the mass spectrometry data, heptose in the oligosaccharide is partially substituted by glycine. H. alvei PCM 2670 core structure encompasses the common core of H. alvei which is modified with two additional galactose units.Figure optionsDownload as PowerPoint slideThe 6-substituted galactose is the O-antigen repeating unit substitution residue. The repeating unit consists of five monosaccharide residues and has the following structure: →2)-β-Galp-(1→6)-α-Glcp-(1→6)-αGlcpNAc3OAc-(1→4)-α-GalpA-(1→3)-β-GlcpNAc6OAc-(1→6)-core.

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