Structures of cell-wall phosphate-containing glycopolymers of Bifidobacterium longum BIM B-476-D

• Сell-wall phosphate-containing glycopolymers of B. longum were isolated.
• A poly(oligosaccharyl phosphate), ribitol and glycerol teichoic acids were identified.
• A GlcNAc/Glc (major/minor) alternation occurs in the poly(oligosaccharyl phosphate).
• The main chain of the ribitol teichoic acid is terminated with d-alanine.

Glycopolymers with oligosaccharyl phosphate repeats of two types and ribitol and glycerol teichoic acids were isolated from cell wall of Bifidobacterium longum BIM B-476-D by stepwise extraction with 10% CCl3CO2H. The following structures of the glycopolymers were established by sugar analysis, selective cleavage with aq 2% HOAc, dephosphorylation with 48% HF, 2D NMR spectroscopy, and high-resolution ESI MS: Figure optionsDownload as PowerPoint slide The ribitol teichoic acid also contains minor d-alanine, whose position was not determined.

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