کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1390413 1500901 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Revision of the O-polysaccharide structure of Yersinia pseudotuberculosis O:1a; confirmation of the function of WbyM as paratosyltransferase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Revision of the O-polysaccharide structure of Yersinia pseudotuberculosis O:1a; confirmation of the function of WbyM as paratosyltransferase
چکیده انگلیسی

An O-polysaccharide was isolated by mild acid degradation at pH 4.5 of the long-chain lipopolysaccharide of Yersinia pseudotuberculosis PB1 (serotype O:1a) and studied using 2D NMR spectroscopy. It was found to contain two uncommon monosaccharides: paratose (3,6-dideoxy-d-ribo-hexose, Par) in the furanose form and 6-deoxy-d-manno-heptose (d-6dmanHep). The following structure of a branched tetrasaccharide repeat (O-unit) with a disaccharide side chain was established:This structure is at variance with the O-polysaccharide structure of Y. pseudotuberculosis O:1a reported earlier (Komandrova, N. A.; Gorshkova, R. P.; Isakov, V. V.; Ovodov, Y. S. Bioorg. Khim.1984, 10, 232–237). A comparative study by high-resolution ESI MS of the short-chain lipopolysaccharides from strain PB1 and a wbyM mutant thereof confirmed the function of wbyM as the paratosyltransferase gene.

Figure optionsDownload as PowerPoint slideHighlights
► Structure of Y. pseudotuberculosis PB1 (O:1a) OPS repeating unit was revised from the published one.
► wbyM mutant was produced from the parent strain.
► Comparative study of LPS structures revealed that wbyM is the paratosyltransferase gene.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 350, 1 March 2012, Pages 98–102
نویسندگان
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