کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1391069 983187 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Type I arabinogalactan contains β-d-Galp-(1→3)-β-d-Galp structural elements
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Type I arabinogalactan contains β-d-Galp-(1→3)-β-d-Galp structural elements
چکیده انگلیسی

Arabinogalactan type I from potato was partially degraded by endo-galactanase from Aspergillus niger. High-performance anion-exchange chromatography revealed that several of the oligomeric degradation products eluted as double peaks. To investigate the nature of these products, the digest was fractionated by Bio-Gel P2 chromatography. The pool that contained tetramers was treated with a β-d-Galp-(1→4)-specific galactosidase from Bifidobacterium adolescentis to obtain a dimer with deviating linkage type, which was further purified by BioGel P2 chromatography. By obtaining all 1H and 13C chemical shifts and the presence of intra residual scalar coupling (HMBC) it could be concluded that the dimer contained a β-(1→3)-linkage instead of the expected β-(1→4)-linkage. Using the same NMR techniques as for the dimer, it was found that the pool of tetramers consisted of the following two galactose tetramers: β-Galp-(1→4)-β-Galp-(1→4)-β-Galp-(1→4)-α/β-Galp-OH and β-Galp-(1→4)-β-Galp-(1→4)-β-Galp-(1→3)-α/β-Galp-OH. The fact that the deviating β-(1→3)-linked galactose was found at the reducing end of the dimer showed that this deviating linkage is present within the backbone. The β-(1→3)-galactosyl interruption appeared to be a common structural feature of type I arabinogalactans with a frequency ranging from approximately 1 in 160 (potato, soy, citrus) to 1 in 250 (onion).

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 340, Issue 13, 26 September 2005, Pages 2135–2143
نویسندگان
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