کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1391310 983241 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dissecting Heterogeneous Molecular Chaperone Complexes Using a Mass Spectrum Deconvolution Approach
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Dissecting Heterogeneous Molecular Chaperone Complexes Using a Mass Spectrum Deconvolution Approach
چکیده انگلیسی

SummarySmall heat-shock proteins (sHSPs) are molecular chaperones that prevent irreversible aggregation through binding nonnative target proteins. Due to their heterogeneity, these sHSP:target complexes remain poorly understood. We present a nanoelectrospray mass spectrometry analysis algorithm for estimating the distribution of stoichiometries comprising a polydisperse ensemble of oligomers. We thus elucidate the organization of complexes formed between sHSPs and different target proteins. We find that binding is mass dependent, with the resultant complexes reflecting the native quaternary architecture of the target, indicating that protection happens early in the denaturation. Our data therefore explain the apparent paradox of how variable complex morphologies result from the generic mechanism of protection afforded by sHSPs. Our approach is applicable to a range of polydisperse proteins and provides a means for the automated and accurate interpretation of mass spectra derived from heterogeneous protein assemblies.


► Heterogeneous complexes of small heat-shock proteins with destabilized targets
► Organization of intact complexes interrogated by means of mass spectrometry
► Mass spectra deconvoluted by using CHAMP, a data analysis algorithm
► Small heat-shock proteins protect targets rapidly and in a mass-dependent manner

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 19, Issue 5, 25 May 2012, Pages 599–607
نویسندگان
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