کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1391315 983241 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tailoring Enzymes Involved in the Biosynthesis of Angucyclines Contain Latent Context-Dependent Catalytic Activities
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Tailoring Enzymes Involved in the Biosynthesis of Angucyclines Contain Latent Context-Dependent Catalytic Activities
چکیده انگلیسی

SummaryComparison of homologous angucycline modification enzymes from five closely related Streptomyces pathways (pga, cab, jad, urd, lan) allowed us to deduce the biosynthetic steps responsible for the three alternative outcomes: gaudimycin C, dehydrorabelomycin, and 11-deoxylandomycinone. The C-12b-hydroxylated urdamycin and gaudimycin metabolites appear to be the ancestral representatives from which landomycins and jadomysins have evolved as a result of functional divergence of the ketoreductase LanV and hydroxylase JadH, respectively. Specifically, LanV has acquired affinity for an earlier biosynthetic intermediate resulting in a switch in biosynthetic order and lack of hydroxyls at C-4a and C-12b, whereas in JadH, C-4a/C-12b dehydration has evolved into an independent secondary function replacing C-12b hydroxylation. Importantly, the study reveals that many of the modification enzymes carry several alternative, hidden, or ancestral catalytic functions, which are strictly dependent on the biosynthetic context.

Graphical AbstractFigure optionsDownload high-quality image (284 K)Download as PowerPoint slideHighlights
► Comparison of tailoring enzymes from five pathways led to synthesis of three products
► The enzymes responsible for divergence of landomycin and jadomycin were identified
► Many of the tailoring enzymes carried alternative, hidden, or ancestral functions
► The study clarified the evolutionary relationships of various angucycline metabolites

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 19, Issue 5, 25 May 2012, Pages 647–655
نویسندگان
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