کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1391519 | 983301 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Generation and Structural Analysis of Reactive Empty Particles Derived from an Icosahedral Virus Generation and Structural Analysis of Reactive Empty Particles Derived from an Icosahedral Virus](/preview/png/1391519.png)
SummaryChemical and genetic modifications on the surface of viral protein cages confer unique properties to the virus particles with potential nano and biotechnological applications. The enclosed space in the interior of the virus particles further increases its versatility as a nanomaterial. In this paper, we report a simple method to generate a high yield of stable cowpea mosaic virus (CPMV) empty capsids from their native nucleoprotein counterparts by removing the encapsidated viral genome without compromising the integrity of the protein coat. Biochemical and structural comparison of artificially generated empty particles did not reveal any distinguishable differences from CPMV particles containing viral RNA. Preliminary results on the use of artificially produced empty CPMV capsids as a carrier capsule are described.
Journal: - Volume 13, Issue 7, July 2006, Pages 771–778