کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1391586 | 983589 | 2010 | 11 صفحه PDF | دانلود رایگان |
SummaryThe development of new anticancer agents derived from natural resources requires a rapid identification of their molecular mechanism of action. To make this step short, we have initiated the proteomic profiling of HeLa cells treated with anticancer drugs representing a wide spectrum of mechanisms of action using two-dimensional difference gel electrophoresis (2D-DIGE). Unique proteome patterns were observed in HeLa cells treated with the HSP90 inhibitor geldanamycin, and were similar to the patterns induced by radicicol, a structurally different HSP90 inhibitor. On the other hand, etoposide and ICRF-193, compounds claimed to be topoisomerase II inhibitors, showed different proteomic profiles, which reflect their different biological activities as revealed by cell-cycle analysis. Thus far, combined data from 19 compounds have allowed their successful classification by cluster analysis according to the mechanism of action.
► Proteomic profiling 2D-PAGE method was developed to allow a classification of small molecules according to the mechanism of action
► Proof of concept showed that proteomic patterns of geldanamycin and radicicol-treated HeLa cells are similar
► Extensive proteomic analysis of HeLa cells treated with compounds of known mechanisms of action established the clustering basis.
Journal: - Volume 17, Issue 5, 28 May 2010, Pages 460–470