کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1391603 | 983594 | 2009 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The E1 Mechanism in Photo-Induced β-Elimination Reactions for Green-to-Red Conversion of Fluorescent Proteins The E1 Mechanism in Photo-Induced β-Elimination Reactions for Green-to-Red Conversion of Fluorescent Proteins](/preview/png/1391603.png)
SummaryKikGR is a fluorescent protein engineered to display green-to-red photoconvertibility that is induced by irradiation with ultraviolet or violet light. Similar to Kaede and EosFP, two naturally occurring photoconvertible proteins, KikGR contains a His62-Tyr63-Gly64 tripeptide sequence, which forms a green chromophore that can be photoconverted to a red one via formal β-elimination and subsequent extension of a π-conjugated system. Using a crystallizable variant of KikGR, we determined the structures of both the green and red state at 1.55 Å resolution. The double bond between His62-Cα and His62-Cβ in the red chromophore is in a cis configuration, indicating that rotation along the His62 Cα-Cβ bond occurs following cleavage of the His62 Nα-Cα bond. This structural rearrangement provides evidence that the β-elimination reaction governing the green-to-red photoconversion of KikGR follows an E1 (elimination, unimolecular) mechanism.
Journal: - Volume 16, Issue 11, 25 November 2009, Pages 1140–1147