کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1391647 | 983611 | 2008 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Proposed Arrangement of Proteins Forming a Bacterial Type II Polyketide Synthase Proposed Arrangement of Proteins Forming a Bacterial Type II Polyketide Synthase](/preview/png/1391647.png)
SummaryAklanonic acid is synthesized by a type II polyketide synthase (PKS) composed of eight protein subunits. The network of protein interactions within this complex was investigated using a yeast two-hybrid system, by coaffinity chromatography and by two different computer-aided protein docking simulations. Results suggest that the ketosynthase (KS) α and β subunits interact with each other, and that the KSα subunit also probably interacts with a malonyl-CoA:ACP acyltransferase (DpsD), forming a putative minimal synthase. We speculate that DpsD may physically inhibit the priming reaction, allowing the choice of propionate rather than acetate as the starter unit. We also suggest a structural role for the cyclase (DpsY) in maintaining the overall structural integrity of the complex.
Journal: - Volume 15, Issue 11, 24 November 2008, Pages 1156–1165