کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1391689 | 983620 | 2008 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Incomplete β-Ketone Processing as a Mechanism for Polyene Structural Variation in the FR-008/Candicidin Complex Incomplete β-Ketone Processing as a Mechanism for Polyene Structural Variation in the FR-008/Candicidin Complex](/preview/png/1391689.png)
SummaryFR-008/candicidin is a heptaene macrolide with established antifungal activity, produced by Streptomyces sp. FR-008 as a complex mixture of compounds. Here, six components (FR-008-I to -VI) of the FR-008/candicidin complex were determined; III, V, and VI were confirmed as natural products, principally differing from each other at C-3 and C-9, while the other three were believed to originate from the respective conversions of the natural ones in vitro. Inactivation of KR21 and DH18, respectively, abolished production of V carrying a C-3 hydroxyl, and VI carrying a C-9 methylene. Combined inactivation created a mutant producing only III, with a C-3 ketone and a C-9 hydroxyl, and having antifungal activity superior to V and comparable to VI. Incomplete activities of KR21 and DH18 were, therefore, unambiguously identified as being involved in structural variations of FR-008 complex.
Journal: - Volume 15, Issue 6, 23 June 2008, Pages 629–638