کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1391701 | 983625 | 2007 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Engineering Unnatural Nucleotide Specificity to Probe G Protein Signaling Engineering Unnatural Nucleotide Specificity to Probe G Protein Signaling](/preview/png/1391701.png)
SummaryG proteins comprise ∼0.5% of proteins encoded by mammalian genomes. To date, there exists a lack of small-molecule modulators that could contribute to their functional study. In this report, we present the use of H-Ras to develop a system that answers this need. Small molecules that allow for the highly specific inhibition or activation of the engineered G protein were developed. The rational design preserved binding of the natural substrates to the G protein, and the mutations were functionally innocuous in a cellular context. This tool can be used for isolating specific G protein effectors, as we demonstrate with the identification of Nol1 as a putative effector of H-Ras. Finally, the generalization of this system was confirmed by applying it to Rap1B, suggesting that this method will be applicable to other G proteins.
Journal: - Volume 14, Issue 9, 21 September 2007, Pages 1007–1018