کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1391732 983630 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
5S Clavam Biosynthetic Genes Are Located in Both the Clavam and Paralog Gene Clusters in Streptomyces clavuligerus
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
5S Clavam Biosynthetic Genes Are Located in Both the Clavam and Paralog Gene Clusters in Streptomyces clavuligerus
چکیده انگلیسی

SummaryThe Streptomyces clavuligerus clavam gene cluster was examined to identify genes specifically involved in 5S clavam biosynthesis. A reduction/loss of 5S clavam production was seen in cvm2 and cvm5 gene mutants, and a clavam metabolite not previously observed, 2-carboxymethylideneclavam, accumulated in the cvm5 mutant. Disruption of additional genes from the region of the clavam cluster did not have any effect on 5S clavam production. Examination of the paralog gene cluster region for 5S clavam biosynthetic genes led to the identification of cvm6P and cvm7P, which encode a putative aminotransferase and a transcriptional regulator, respectively. Mutants defective in cvm6P and cvm7P were completely blocked in 5S clavam but not clavulanic acid production. The loss of 5S clavam production in cvm7P mutants suggests that this gene encodes a transcriptional regulator specific for 5S clavam metabolite biosynthesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 14, Issue 2, February 2007, Pages 131–142
نویسندگان
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