کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1391749 983632 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural Basis for Carbapenemase Activity of the OXA-23 β-Lactamase from Acinetobacter baumannii
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Structural Basis for Carbapenemase Activity of the OXA-23 β-Lactamase from Acinetobacter baumannii
چکیده انگلیسی


• OXA-23 is the major carbapenemase in Acinetobacter
• Antibiotic susceptibility and enzyme kinetics of OXA-23 have been determined
• The crystal structure of OXA-23 has been solved by X-ray crystallography
• The structure of a wild-type CHDL complexed with a carbapenem substrate is presented

SummaryDissemination of Acinetobacter baumannii strains harboring class D β-lactamases producing resistance to carbapenem antibiotics severely limits our ability to treat deadly Acinetobacter infections. Susceptibility determination in the A. baumannii background and kinetic studies with a homogeneous preparation of OXA-23 β-lactamase, the major carbapenemase present in A. baumannii, document the ability of this enzyme to manifest resistance to last-resort carbapenem antibiotics. We also report three X-ray structures of OXA-23: apo OXA-23 at two different pH values, and wild-type OXA-23 in complex with meropenem, a carbapenem substrate. The structures and dynamics simulations reveal an important role for Leu166, whose motion regulates the access of a hydrolytic water molecule to the acyl-enzyme species in imparting carbapenemase activity.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 20, Issue 9, 19 September 2013, Pages 1107–1115
نویسندگان
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