کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1391925 | 983666 | 2011 | 14 صفحه PDF | دانلود رایگان |
SummaryA single gene cluster of Penicillium chrysogenum contains genes involved in the biosynthesis and secretion of the mycotoxins roquefortine C and meleagrin. Five of these genes have been silenced by RNAi. Pc21g15480 (rds) encodes a nonribosomal cyclodipeptide synthetase for the biosynthesis of both roquefortine C and meleagrin. Pc21g15430 (rpt) encodes a prenyltransferase also required for the biosynthesis of both mycotoxins. Silencing of Pc21g15460 or Pc21g15470 led to a decrease in roquefortine C and meleagrin, whereas silencing of the methyltransferase gene (Pc21g15440; gmt) resulted in accumulation of glandicolin B, indicating that this enzyme catalyzes the conversion of glandicolin B to meleagrin. All these genes are transcriptionally coregulated. Our results prove that roquefortine C and meleagrin derive from a single pathway.
► A single gene cluster encodes the biosynthesis of two different mycotoxins
► Roquefortine and meleagrin are formed by a nonribosomal cyclodipeptide synthetase
► The His-Trp cyclodipeptide is prenylated by a reverse prenyltransferase
► The mutant silenced in the methyltransferase (gmt) accumulates glandicolin B
Journal: - Volume 18, Issue 11, 23 November 2011, Pages 1499–1512