A Synthetic Photoactivated Protein to Generate Local or Global Ca2+ Signals

SummaryCa2+ signals regulate diverse physiological processes through tightly regulated fluxes varying in location, time, frequency, and amplitude. Here, we developed LOVS1K, a genetically encoded and photoactivated synthetic protein to generate local or global Ca2+ signals. With 300 ms blue light exposure, LOVS1K translocated to Orai1, a plasma membrane Ca2+ channel, within seconds, generating a local Ca2+ signal on the plasma membrane, and returning to the cytoplasm after tens of seconds. With repeated photoactivation, global Ca2+ signals in the cytoplasm were generated to modulate engineered Ca2+-inducible proteins. Although Orai1 is typically associated with global store-operated Ca2+ entry, we demonstrate that Orai1 can also generate local Ca2+ influx on the plasma membrane. Our photoactivation system can be used to generate spatially and temporally precise Ca2+ signals and to engineer synthetic proteins that respond to specific Ca2+ signals.

Graphical AbstractFigure optionsDownload high-quality image (82 K)Download as PowerPoint slideHighlights
► Developed LOVS1K, a genetically encoded and light-controllable synthetic protein
► Reversibly and specifically activates Orai1 Ca2+ channels
► Can generate both local or global Ca2+ signals with repeated photostimulation
► Used LOVS1K to modulate previously reported engineered Ca2+-inducible proteins