کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1392477 | 983743 | 2006 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Metabolic Engineering of Pseudomonas putida for Methylmalonyl-CoA Biosynthesis to Enable Complex Heterologous Secondary Metabolite Formation Metabolic Engineering of Pseudomonas putida for Methylmalonyl-CoA Biosynthesis to Enable Complex Heterologous Secondary Metabolite Formation](/preview/png/1392477.png)
SummaryAn operon consisting of three open reading frames, annotated in silico as methylmalonyl-CoA (mm-CoA) epimerase, mm-CoA mutase (MCM), and meaB, was identified in the sequencing project of the myxobacterium Sorangium cellulosum So ce56. This putative MCM pathway operon was subcloned from a bacterial artificial chromosome by Red/ET recombineering onto a minimal replicon derived from p15A. This plasmid was modified for integration and heterologous expression in Pseudomonas putida to enable the production of complex secondary metabolites requiring mm-CoA as precursor. Methylmalonate was identified in the recombinant P. putida strain by an analysis method based on gas chromatography/mass spectrometry. The engineered strain is able to synthesize polyketides requiring mm-CoA as an extender unit, which was demonstrated by the production of myxothiazol after integration of the biosynthetic gene cluster into the chromosome, followed by induction of expression.
Journal: - Volume 13, Issue 12, December 2006, Pages 1253–1264