کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1392695 | 983765 | 2008 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The Importance of Odorant Conformation to the Binding and Activation of a Representative Olfactory Receptor The Importance of Odorant Conformation to the Binding and Activation of a Representative Olfactory Receptor](/preview/png/1392695.png)
SummaryOlfactory receptors (ORs) form a large family of G protein-coupled receptor proteins (GPCRs) responsible for sensing the ambient chemical environment. The molecular recognition strategies used by ORs to detect and distinguish odorant molecules are unclear. Here, we investigated the variable of odorant carbon chain conformation for an established odorant-OR pair: n-octanal and rat OR-I7. A series of conformationally restricted octanal mimics were tested on live olfactory sensory neurons (OSNs). Our results support a model in which unactivated OR-I7 binds aliphatic aldehydes indiscriminately, and then applies conformational and length filters to distinguish agonists from antagonists. Specific conformers are proposed to activate OR-I7 by steric buttressing of an OR activation pocket. Probing endogenously expressed rat OSNs with octanal and constrained mimics furnished evidence that odorant conformation contributes to an odorant's unique olfactory code signature.
Journal: - Volume 15, Issue 12, 22 December 2008, Pages 1317–1327