کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1394204 | 984042 | 2006 | 13 صفحه PDF | دانلود رایگان |
SummaryPhenalinolactones are terpene glycosides with antibacterial activity. A striking structural feature is a highly oxidized γ-butyrolactone of elusive biosynthetic origin. To investigate the genetic basis of the phenalinolactones biosynthesis, we cloned and sequenced the corresponding gene cluster from the producer strain Streptomyces sp. Tü6071. Spanning a 42 kbp region, 35 candidate genes could be assigned to putatively encode biosynthetic, regulatory, and resistance-conferring functions. Targeted gene inactivations were carried out to specifically manipulate the phenalinolactones pathway. The inactivation of a sugar methyltransferase gene and a cytochrome P450 monoxygenase gene led to the production of modified phenalinolactone derivatives. The inactivation of a Fe(II)/α-ketoglutarate-dependent dioxygenase gene disrupted the biosynthetic pathway within γ-butyrolactone formation. The structure elucidation of the accumulating intermediate indicated that pyruvate is the biosynthetic precursor of the γ butyrolactone moiety.
Journal: - Volume 13, Issue 4, April 2006, Pages 365–377