کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1394206 | 984042 | 2006 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Functional Analysis of the Validamycin Biosynthetic Gene Cluster and Engineered Production of Validoxylamine A Functional Analysis of the Validamycin Biosynthetic Gene Cluster and Engineered Production of Validoxylamine A](/preview/png/1394206.png)
SummaryA 45 kb DNA sequencing analysis from Streptomyces hygroscopicus 5008 involved in validamycin A (VAL-A) biosynthesis revealed 16 structural genes, 2 regulatory genes, 5 genes related transport, transposition/integration or tellurium resistance; another 4 genes had no obvious identity. The VAL-A biosynthetic pathway was proposed, with assignment of the required genetic functions confined to the sequenced region. A cluster of eight reassembled genes was found to support VAL-A synthesis in a heterologous host, S. lividans 1326. In vivo inactivation of the putative glycosyltransferase gene (valG) abolished the final attachment of glucose for VAL production and resulted in accumulation of the VAL-A precursor, validoxylamine, while the normal production of VAL-A could be restored by complementation with valG. The role of valG in the glycosylation of validoxylamine to VAL-A was demonstrated in vitro by enzymatic assay.
Journal: - Volume 13, Issue 4, April 2006, Pages 387–397