Functionalized carbon nanotube-bienzyme biocomposite for amperometric sensing

An approach to design a biocomposite bienzyme biosensor with the aim of evaluating its suitability as an amperometric sensor using functionalized multiwalled carbon nanotubes (MWCNTs) is presented. The biosensor is based on a bienzyme-channelling configuration, employing the enzymes glucose oxidase (GOx) and horseradish peroxidase (HRP), which were immobilized with toluidine blue (TB) functionalized MWCNTs. The proposed method demonstrates an easy electron transfer between the immobilized enzymes and the electrode via functionalized MWCNTs in a Nafion matrix. Co-immobilization of GOx and HRP was employed to establish the feasibility of fabricating highly effective bienzyme-based biosensors for low-level glucose determination. Bienzyme immobilized TB functionalized MWCNTs were attached to a glassy carbon electrode, and the electrochemical behavior of the sensor was studied using electrochemical impedance spectroscopy, cyclic voltammetry and chronoamperometry. The excellent electrocatalytic activity of the biocomposite film resulted in the detection of glucose under reduced over potential with a wider range of determination from 1.5 × 10−8 M to 1.8 × 10−3 M and with a detection limit of 3 × 10−9 M. The sensor showed a short response time (within 2 s), good stability and anti-interferant ability. The proposed biosensor exhibits good analytical performance in terms of repeatability, reproducibility and shelf-life stability.