Graphene–gold nanoparticle composite: Application as a good scaffold for construction of glucose oxidase biosensor

• PGRO–AuNPs modified electrode employed as a reliable scaffold for GODx immobilization.
• AuNPs prevent stacking PRGO layers, thus improve the electrochemical behavior of biosensor.
• GODx electron transfer was improved because of good interaction with PRGO–AuNP scaffold.
• PRGO–AuNP/GODx modified biosensor showed excellent sensitivity towards glucose.

In the present work we report a facile method for fabrication of glucose oxidase immobilized on the partially reduced graphene–gold nanocomposite (PRGO–AuNPs/GOx) as a novel biosensor for determination of glucose concentration. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to study the morphology of PRGO and PRGO–AuNPs. Also, fast Fourier transformation infrared spectroscopy (FTIR) and UV–Vis spectroscopy were used to confirm formation of graphene and graphene–gold composite. Then, the electrochemical behavior of PRGO–AuNPs/GOx modified electrode was studied by cyclic voltammetry (CV). Our electrochemical studies, especially chronoamperometry (CA), showed that the PRGO–AuNPs/GOx modified electrode has excellent electrocatalytic activity towards the glucose. The limit of detection and sensitivity towards glucose were estimated as 0.06 μM and 15.04 mA mM− 1, respectively.

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